Antibodies & immunoassays for small molecules Histamine ELISA kit
High Sensitivity I Plasma & Urine
Ref: BA-E-1000
NeuromediatorsHistamineDiagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISA
+Plasma/Urine Histamine ELISA
DatasheetMSDSProtocol
This Histamine ELISA kit is tailored for the quantitative measurement of histamine in urine and plasma samples. Intended for in vitro diagnostics & research, the immunoassay can be combined with the add-on kit BA-E-1100, to quantify histamine release in whole blood.
| Sample type | 10µl urine, 25µl plasma |
| Samples/kit | 40 samples in duplicate |
| Sensitivity | 0.3ng/ml urine, 0.12ng/ml plasma |
| Range | 0.5 – 50ng/ml |
| Assay time | Sample acylation (1h) and ELISA overnight |
| Reactivity | Reacts with all species |
| Citations | Cited in 2 papers |
Product overview
| Product name | Histamine ELISA kit – Urine and Plasma samples |
| Description | In vitro diagnostics & research enzyme immunoassay (ELISA) for the quantitative determination of histamine levels in urine and plasma samples |
| Labels | IVD, CE |
| Format | 96-well plate |
| Samples | 10µl urine, 25µl plasma |
| Reactivity | Reacts with all species |
| Standard range | 0 / 0.5 – 50ng/ml |
| Sensitivity |
0.12ng/ml plasma, 0.3ng/ml urine |
| Specificity | No significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin |
| Assay time | 1h sample acetylation + ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material safety datasheet |
| Sample collection & storage | Plasma: EDTA or Heparin plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C
Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C |
| Sample preparation | Sample acylation (1h) |
| ELISA | Histamine antiserum incubation (3H or overnight), revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Product citations
- Increased serum diamine oxidase activity in nonallergic patients with migraine
Check the article
Authors : Garcia-Martin et al, European journal of Clinical Investigation
2022-02 - Inactivated antithombin as anticoagulant reversal in a rat model of cardiopulmonary bypass: a potent and potentially safer alternative to protamine
Check the article
Authors : Bianchini et al., Britis journal of Haematology
2018-01
