Histamine release kit
Whole blood samples
Ref: BA-E-1100
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Histamine release add-on kit
DatasheetProtocolMSDS
Intended for in vitro diagnostics & research, this add-on kit to the BA-E-1000 histamine ELISA kit enables the measurement of histamine release in heparinized whole blood samples, allowing for instance to determine the sensitivity of basophilic granulocytes to specific allergens.
Sample type | 500µl whole blood |
Samples/kit | 40 samples in duplicate |
Sensitivity | 0.2ng/ml |
Range | 0.5 – 50ng/ml |
Assay time | Sample preparation (1h30) |
Reactivity | Reacts with all species |
Product overview
Product name | Histamine release kit – Whole blood samples |
Description | Supplementary kit to in vitro diagnostics enzyme immunoassay (ELISA) for the quantitative determination of histamine release in whole blood samples |
Labels | IVD, CE |
Format | 96-well plate |
Samples | 500µl whole blood |
Reactivity | Reacts with all species |
Standard range | 0 / 0.5 – 50ng/ml |
Sensitivity |
0.2ng/ml |
Specificity | No significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin |
Assay time | 1h30 sample preparation + ELISA overnight |
Storage | Store at 2-8°C for up to 6 months |
Datasheets | Instructions for use, Material safety datasheet |
Sample collection & storage | 24-hours heparinized whole blood stored at 20-25°C |
Sample preparation | Sample acylation (1h30) |
ELISA | Histamine antiserum incubation (3H or overnight), revelation and read steps (1h) |
Detailed protocol | Download instructions for use |
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