Antibodies & immunoassays for small molecules STAINperfect™ – Trial pack
Ref: SP-T-0001
IFStaining kits
+GABAergic network and glutamatergic cell populations
in a mature rat cortical culture revealed by GABA and L-glutamate immunoreactivity detection using mouse monoclonal (#IS039) and rabbit polyclonal (#IS1001) antibodies, respectively, with the STAINperfect immunostaining kit A.
DatasheetMSDSProtocol
The trial pack STAINperfect™ allows testing the STAINperfect™ immunostaining kit A in combination with 2 primary antibodies to key neurotransmitters and small molecules. The kit is provided with all necessary reagents and protocols to test the STAINperfect™ procedure in cell cultures, whole mounts and brain tissue sections.
| Contents | 1x STAINperfect™ immunostaining kit A 2x 25µl antibody vials |
| Number of stainings | Cell cultures : 1x 96 well-plate Whole mounts : 20 samples Tissue sections: 40 slides |
| References | Cited in 15 papers |
They published with this product see papers
Product overview
| Product name | STAINperfect™ Neurotransmiters – Immunostaining Trial Pack |
| Description | Trial offer consisting of a STAINperfect™ immunostaining kit A and 2 primary antibodies in 25µl format. |
| Eligible samples | Cell cultures, whole mounts, tissue sections (any species) |
| Number of stainings | Cell cultures : 1x 96 well-plate Whole mounts : 20 samples Tissue sections: 40 slides |
| Storage |
Store at 4C° until use |
| Restriction | For research use only |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibodies with the antibody diluent provided in the STAINperfect™ immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect™ protocol suited to your sample. |
| Comments | Optimal working dilutions must be determined by the end-user. Third-party anti-rabbit secondary antibody required. |
| Protocols |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
Product citations
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2020-07 - Reproducible generation of human midbrain organoids for in vitro modeling of Parkinson’s disease
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Authors : Nickels et al., Stem Cell Research
2020-06 - Impact of Sleep-Wake-Associated Neuromodulators and Repetitive Low-Frequency Stimulation on Human iPSC-Derived Neurons
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Authors : Yokoi et al., Frontiers in neuroscience
2019-05 - Modeling Parkinson’s disease in midbrain-like organoids
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Authors : Smits et al., NPJ Parkinson's disease
2019-04 - hiPS Cell-Derived Neurons for High-Throughput Screening
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Authors : Traub et al., Methods in molecular biology
2019-01 - miR-34b/c Regulates Wnt1 and Enhances Mesencephalic Dopaminergic Neuron Differentiation
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2018-03 - Detection of the Cyanotoxins L-BMAA Uptake and Accumulation in Primary Neurons and Astrocytes
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Authors : Tan et al, Neurotoxicity research
2017-08 - Derivation of Human Midbrain-Specific Organoids from Neuroepithelial Stem Cells
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Authors : Monzel et al., Step cell reports
2017-05
