Reverse T3 (rT3) ELISA kit, Fast, Serum & Plasma

Ref: TF-E-2500
Diagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISAEndocrinology
Reverse T3 (rT3) ELISA TF-E-2500+
Reverse T3 (rT3) ELISA TF-E-2500

Reverse T3 (rT3) ELISA TF-E-2500
Reverse T3 (rT3) ELISA TF-E-2500
DatasheetMSDSProtocol (IVD) Protocol (RUO)

This Reverse T3 ELISA kit is intended for the quantitative measurement of reverse Triiodothyronine (rT3) in serum and plasma samples (EDTA, lithium heparin, or citrate) to assess thyroid function. Designed for both in vitro diagnostic and research applications, this fast and reliable assay (105 minutes) utilizes a 96-well plate format, making it ideal for high-throughput screening with quick turnaround times.

 

Sample type EDTA, lithium heparin or citrate serum or plasma
Capacity 96 tests
Sensitivity 0.014 ng/mL
Range 0/0.02 – 2 ng/mL
Assay time 105 min
Reactivity Reacts with all species

 

Size

1 x 96 wells
Regulatory status

In Vitro Diagnostics (EU), Research Use Only

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Product name Reverse T3 (rT3) ELISA
Description In vitro diagnostics / Research enzyme immunoassay (ELISA) for the quantitative determination of Reverse Triiodothyronine in serum and plasma samples.
Labels IVD (EU only) or RUO, CE
Format 96-well plate
Samples Serum, Plasma (EDTA, lithium heparin, or citrate)
Sample volume 25µL
Reactivity Reacts with all species
Standard range 0/0.02 – 2 ng/mL
Sensitivity

0.014 ng/mL
Specificity No significant cross-reactivity was observed with Reverse Triiodothyronine (rT3) such as Thyroxine (T4),  triiodothyronine (T3) [3,3`,5-triiodothyronine], 3,5-Diiodo-L-thyronine (T2).
Assay time 95 min preparation, 5-10 min  ELISA

Sample collection & storage Store at 2-8°C for up to 6 months

Plasma/Serum: EDTA-, lithium- heparin-, citrate- samples stored at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). Do not use haemolytic, icteric, or lipemic samples
Sample preparation Reverse T3-Biotin conjugation (60 min) and Streptavidin-HRP conjugate conjugation (80 min)
ELISA Incubation, revelation and read steps (10 to 20 min)
Detailed protocol RUO: Download instructions for use

IVD: Download protocol for IVD

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