Ornithine ELISA kit – High Sensitivity – Plasma samples

Ref: IS-I-1000R
Arginine metabolismAmino acidsResearch ELISA kits (RUO)ELISA
DatasheetProtocolMSDS

Free L-Arginine (Arg) is provided by diet, de novo synthesis and protein turnover. De novo synthesis is sufficiently important to not consider Arg as an essential amino acid. However, in some circumstances – eg. early development, infection and inflammation – de novo synthesis is not enough and require dietary Arg, which is thus classified as a conditionally-essential amino acid. Whatever the source, Arg can be metabolized through different degrading pathways which include i) the nitric oxide synthase (NOS) pathway providing Citrulline and NO, as well as ii) the well-known Arginase pathway leading to the production of Urea and L-Ornithine (Orn).

 

Sample type Plasma
Capacity 96 tests
Sensitivity 8.5µM
Range 20 – 781.25µM
Assay time Sample preparation 3h, ELISA overnight
Reactivity Reacts with all species
References Cited in 1 paper

 

Size

1 x 96 wells

539.00

82 in stock

Quantity
Total

539.00

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Product overview

Product name L-Ornithine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of L-Ornithine (Orn) in plasma samples. For research use only
Format 96-well plate
Samples Plasma
Minimal sample volume 20µL
Reactivity Reacts with all species
Standard range 20 – 781.25µM
Sensitivity

8.5µM

Specificity No significant cross-reactivity was observed with Agmatine, L-Arginine, NG-Methyl-L-Arginine, L-Argino-succinate, L-Citrulline, L-Glutamine & L-Glutamate
Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months
Datasheets Instructions for useMaterial Safety Datasheet

 

For research use only  – Do not use for diagnostic

Sample collection & storage EDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)

Sample preparation Sample preparation (3 hours)
ELISA L-Ornithine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocol Download instructions for use

Product citation

  • Lactobacillus maintains healthy gut mucosa by producing L-Ornithine
    Check the article
    Authors : Qi et al., Communications Biology
    2019-05

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