Antibodies & immunoassays for small molecules Normetanephrine ELISA kit
Fast – Urine Samples
Ref: BA-E-8500
CatecholaminesDiagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISA
+Urine Normetanephrine ELISA
DatasheetProtocolMSDS
This Normetanephrine ELISA kit enables to quantify normetanephrine in urine samples within 2 hours. Intended for in vitro diagnostics & research use, this quantitative immunoassay demonstrated good correlation with HPLC.
| Sample type | 25µl urine |
| Samples/kit | 40 samples in duplicate |
| Sensitivity | 23ng/ml |
| Range | 30 – 3 000ng/ml |
| Assay time | Sample hydrolysis and acetylation (45 min), ELISA (1h) |
| Reactivity | Reacts with all species |
| Citations | Cited in 1 paper |
Product overview
| Product name | Normetanephrine ELISA kit – Urine- Fast-Track |
| Description | In vitro diagnostics & Research-use enzyme immunoassay (ELISA) for the fast determination of normetanephrine (normetadrenaline) in urine samples |
| Labels | IVD (EU only) or RUO, CE |
| Format | 96-well plate |
| Samples | Urine |
| Sample volume | 25µL |
| Reactivity | Reacts with all species |
| Standard range | 0 / 30 – 3 000ng/ml |
| Sensitivity |
23ng/ml |
| Specificity | No significant cross-reactivity was observed with Normetanephrine analogs such as metanephrine, 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, Vanillic mandelic acid, L-Dopa, Homovanillic acid, L-Tyrosine and Tyramine |
| Assay time | Sample hydrolysis and acetylation 45 min and ELISA 1h |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use, Material safety datasheet |
| Sample collection & storage | Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage at -20°C. for long periods (up to 6 months) |
| Sample preparation | Sample hydrolysis (30 min) and acetylation (15min) |
| ELISA | Normetanephrine antiserum incubation (30 min), revelation and read steps (30h) |
| Detailed protocol | Download instructions for use |
Product citation
- Quantification of normetanephrine in canine urine using ELISA: evaluation of factors affecting results
Check the article
Authors : Hoglund et al., Journal of veterinary diagnosic investigation
2021-10
