Antibodies & immunoassays for small molecules N8-Acetyl-Spermidine Antibody – Rabbit Polyclonal
Ref: IS1071
IFIHCArginine metabolismPolyclonal AntibodiesELISAICC
+Affinity & specificity of anti-N8-Acetyl-Spermidine antibody
Competitive ELISA demonstrates that low amounts of N8-Acetyl-Spermidine conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of N1-Acetyl-Spermidine, Spermidine, Spermine, Ornithine, and Putrescine conjugates do not affect reaction (high specificity).
DatasheetMSDS
The anti-N8-Acetyl-Spermidine antibody IS1071 is to date the first and only antibody that allows the detection of the polyamine N8AS in biological samples. Our team is currently working on the IF/ICC/IHC protocol for optimal staining in combination with the STAINperfect immunostaining kit A.
| Clonality | Polyclonal |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Validated by | ELISA |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µl |
Product overview
| Product name | N8-Acetyl-Spermidine antibody- Rabbit pAb |
| Synonyms | Anti-N8acetylspermidine antibody
N8AS antibody Anti-N(8)-acetylspermidine antibody |
| Immunogen | Conjugated N8-Acetyl-Spermidine |
| Specificity | When tested in competitive ELISA, the anti-conjugated L-N8-Acetyl-Spermidine antibody did not show any significant cross-reactivity with N8-Acetyl-Spermidine competitors (see graph). |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage | Store at 4°C |
| Storage buffer | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
Optimization of staining protocol ongoing
| Standard STAINperfect procedure | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
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