Antibodies & immunoassays for small molecules Melatonin ELISA kit
Highly Sensitive – Plasma & Serum Samples
Ref: BA-E-3300
Diagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISANeuromediatorsTryptophan metabolism
+Highly sensitive Melatonin ELISA kit
DatasheetMSDSProtocol
The Melatonin ELISA kit allows for the easy and accurate quantification of N-acetyl-5-methoxy-tryptamine (melatonin) in plasma and serum samples. With a detection limit of 1.6 pg/mL, the kit enables the measurement of melatonin in blood during both daytime (0 to 20 pg/mL) and nighttime (40 to 100 pg/mL).
| Sample type | 500µl plasma or serum |
| Samples/kit | 40 samples in duplicate |
| Sensitivity | 1,6pg/ml plasma |
| Range | 0/3 – 300 pg/ml |
| Assay time | Sample extraction (15 min), incubation (overnight), ELISA (3h) |
| Reactivity | Reacts with all species |
| Material required | Evaporator centrifuge (Speed-Vac) or sample concentrator by use of nitrogen (e.g. Techne) |
Product overview
| Product name | Melatonin ELISA kit – Highly Sensitive – Plasma & Serum Samples |
| Description | In vitro diagnostics & Research enzyme immunoassay (ELISA) for the sensitive determination of Melatonin levels in plasma and serum samples |
| Labels | IVD, CE, RUO |
| Format | 96-well plate |
| Samples | 500µl plasma or serum |
| Reactivity | Reacts with all species |
| Standard range | 0 / 3 – 300 pg/ml |
| Sensitivity |
1,6 pg/ml plasma |
| Specificity | No significant cross-reactivity was observed with Melatonin analogs such as 5-Methoxy-Tryptophole, N-Acetyl-Serotonin, 5-Methoxy-Tryptamine |
| Assay timehttps://www.immusmol.com/wp-content/uploads/2024/08/IFU-BA-E-33 | Sample extraction (15 min), incubation (overnight), ELISA (3h) |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material safety datasheet |
| Sample collection & storage | EDTA or Heparin plasma or serum samples only. Do not use haemolytic, icteric or lipemic samples. Storage: up to 24 hours at 2 – 8°C; for longer periods (up to 3 months) at – 20°C |
| Sample preparation | Sample preparation, extraction and acylation (15min) |
| ELISA | Melatonin antiserum incubation (overnight), revelation and read steps (3h) |
| Detailed protocol | Download instructions for use |
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