Antibodies & immunoassays for small molecules L-Tryptophan Antibody – Mouse Monoclonal
Ref: IS011
Kynurenine pathwayTryptophan metabolismAmino acidsMonoclonal AntibodiesICCIFIHC
+Tryptophan detection in the crayfish brain
Anti-Tryptophan antibody demonstrates the presence of Tryptophan positive cells in the brain of a crayfish. Staining was obtained with mouse monoclonal anti-Tryptophan antibody (IS011) and performed with STAINperfect immunostaining kit A, following the protocol for whole mount samples. Fluorescent conjugated secondary antibody was used and images obtained by confocal imaging at high magnification (x40).
DatasheetMSDS
The anti-Tryptophan antibody IS011 is a mouse monoclonal antibody optimized to ensure high affinity and specificity. Combined with the STAINperfect immunostaining kit A, this antibody allows direct L-Tryptophan visualization in whole mounts, cell cultures, and tissue sections.
| Clonality | Monoclonal antibody (clone 4H11-A11) |
| Host | Mouse |
| Reactivity | Reacts with all species |
| Eligible samples | Whole mounts, cell cultures, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL (approx. 40 tissue sections) |
| References | Cited in 2 papers |
Product overview
| Product name | L-Tryptophan antibody |
| Synonyms | Anti-L-Trp antibody
(S)-2-Amino-3-(3-indolyl)propionic acid antibody L-alpha-Amino-3-indolepropionic acid antibody (S)-2-Amino-3-(3-indolyl)propanoic acid antibody L-alpha-Amino-3-indolepropanoic acid antibody |
| Immunogen | Conjugated L-Tryptophan |
| Isotype | IgG1 k chain |
| Specificity | When tested in competitive ELISA, the anti-conjugated L-Tryptophan antibody did not show any significant cross reactivity with Tryptophan analogs, including Tryptamine |
| Volume | 50µL |
Storage
| Form | Liquid |
| Purity | Purified IgG |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for dopamine staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
Production citations
- Cilia-deficient renal tubule cells are primed for injury with mitochondrial defects and aberrant tryptophan metabolism
Check the article
Authors : Zuo X et al., Am J Physiol Renal Physiol.
2024-07 - Tryptophan As a New Member of RNA-Induced Silencing Complexes Prevents Colon Cancer Liver Metastasis
Check article
Authors : Xu et al., Adv Sci
2024-06
