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L-Tryptophan ELISA kit
Any sample I High Sensitivity
Ref: BA-E-2700Amino acidsDiagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISATryptophan metabolism
+Tryptophan ELISA kit
DatasheetMSDSIVD ProtocolRUO Protocol
This Tryptophan ELISA kit enables the quantitative measurement of L-Tryptophan in biological fluids and cell culture supernatants. Intended for in vitro diagnotics and research applications, the test comes in 96-well plate format, allowing the screening of large experimental series within short lead times.
| Sample type | Biological fluids (urine, plasma, serum, …) and cell culture supernatant |
| Capacity | 96 tests |
| Sensitivity | < 1.2µg/ml |
| Range | 2.5 – 250µg/ml |
| Assay time | 2h30 sample preparation and ELISA overnight |
| Reactivity | Reacts with all species |
| References | Cited in >40 papers |
Product overview
| Product name | Tryptophan ELISA kit |
| Description | In vitro diagnostics / Research enzyme immunoassay (ELISA) for the quantitative determination of L-Tryptophan in biological fluids (urine, plasma, serum samples, …) and cell culture supernatants |
| Labels | IVD, CE |
| Format | 96-well plate |
| Samples | urine, plasma, serum, cell culture supernatant |
| Sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | 2.5 – 250µg/ml |
| Sensitivity | < 1.2µg/ml |
| Specificity | No significant cross-reactivity was observed with L-Tryptophan analogs such as 5-Hydroxy-L-tryptophan, Tryptamine, 5-Methoxytryptophan, 5-Hydroxytryptamine, N-acetyl-5-hydroxytryptamine |
| Assay time | Sample preparation 2.5 hours and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months |
| Datasheets | Instructions for use , Material safety datasheet |
| Sample collection & storage | Urine: spontaneous or 24-hour urine collected in bottle with 10-15 mL of 6 M HCl, stored at -20°C for long periods (up to 6 months) Plasma/Serum: EDTA-, Heparin- or Citrate- samples stored at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). Do not use haemolytic or lipemic samples |
| Sample preparation | Sample precipitation (15 min) and derivatization (2h15) |
| ELISA | Tryptophan antiserum overnight incubation, revelation and read steps (1h) |
| Detailed protocol | IVD: Download instructions for use |
Product citation
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2016-12
