Antibodies & immunoassays for small molecules L-Tryptophan ELISA kit
Any sample I High Sensitivity
Ref: BA-E-2700
Tryptophan metabolismAmino acidsDiagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISA
+Tryptophan ELISA kit
DatasheetMSDSIVD ProtocolRUO Protocol
This Tryptophan ELISA kit enables the quantitative measurement of L-Tryptophan in biological fluids and cell culture supernatants. Intended for in vitro diagnotics and research applications, the test comes in 96-well plate format, allowing the screening of large experimental series within short lead times.
| Sample type | Biological fluids (urine, plasma, serum, …) and cell culture supernatant |
| Capacity | 96 tests |
| Sensitivity | < 1.2µg/ml |
| Range | 2.5 – 250µg/ml |
| Assay time | 2h30 sample preparation and ELISA overnight |
| Reactivity | Reacts with all species |
| References | Cited in 54 papers |
They published with this product see papers
Product overview
| Product name | Tryptophan ELISA kit |
| Description | In vitro diagnostics / Research enzyme immunoassay (ELISA) for the quantitative determination of L-Tryptophan in biological fluids (urine, plasma, serum samples, …) and cell culture supernatants |
| Labels | IVD (EU only) or RUO, CE |
| Format | 96-well plate |
| Samples | urine, plasma, serum, cell culture supernatant |
| Sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | 2.5 – 250µg/ml |
| Sensitivity |
< 1.2µg/ml |
| Specificity | No significant cross-reactivity was observed with L-Tryptophan analogs such as 5-Hydroxy-L-tryptophan, Tryptamine, 5-Methoxytryptophan, 5-Hydroxytryptamine, N-acetyl-5-hydroxytryptamine |
| Assay time | Sample preparation 2.5 hours and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months |
| Datasheets | Instructions for use, Material safety datasheet |
| Sample collection & storage | Urine: spontaneous or 24-hour urine collected in bottle with 10-15 mL of 6 M HCl, stored at -20°C for long periods (up to 6 months)
Plasma/Serum: EDTA-, Heparin- or Citrate- samples stored at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). Do not use haemolytic or lipemic samples |
| Sample preparation | Sample precipitation (15 min) and derivatization (2h15) |
| ELISA | Tryptophan antiserum overnight incubation, revelation and read steps (1h) |
| Detailed protocol | IVD: Download instructions for use |
Product citations
- Continuous theta burst stimulation for bipolar depression: A multicenter, double-blind randomized controlled study exploring treatment efficacy and predictive potential of kynurenine metabolites
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Authors : Dellink et al., Journal of Affective Disorders
2024-09 - Transcriptomic evidence of immune modulation in subjects with chronic Trypanosoma cruzi infection
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Authors : Ros-Lucas et al., J Infect Dis.
2024-08 - Surface-Enhanced Raman Scattering Monitoring of Tryptophan Dynamics in 3D Pancreatic Tumor Models
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Authors : Valera et al., ACS Sensors
2024-07 - VEEV TC-83 Triggers Dysregulation of the Tryptophan–Kynurenine Pathway in the Central Nervous System That Correlates with Cognitive Impairment in Tg2576 Mice
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Authors : Fongsaran C. et al., Pathogens
2024-05 - Effects of a flavonoid-rich blackcurrant beverage on markers of the gut-brain axis in healthy females: Secondary findings from a 4-week randomized crossover control trial
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Authors : Gillies et al., Current Developments in Nutrition
2024-04 - Derangements of immunological proteins in HIV-associated diffuse large B-cell lymphoma: the frequency and prognostic impact
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Authors : Vaughan et al., Front. Cell. Infect. Microbiol.
2024-04 - PABPC1L induces IDO1 to promote tryptophan metabolism and immune suppression in renal cell carcinoma
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Authors : Shu G. et al, Cancer Res
2024-02 - The dangerous “West Coast Swing” by hyperglycaemia and chronic stress in the mouse hippocampus: Role of kynurenine catabolism
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Authors : Gliozzi M et al., Pharmacological Research
2024-01 - Camptothesome-based combination nanotherapeutic regimen for improved colorectal cancer immunochemotherapy
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Authors : Wang Z. et al., Biomaterials
2024-01 - Dietary Marine Hydrolysate Improves Memory Performance and Social Behavior through Gut Microbiota Remodeling during Aging
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Authors : Mougin et al., Foods
2023-11 - Sphingomyelin-derived nanovesicles for the delivery of the IDO1 inhibitor epacadostat enhance metastatic and post-surgical melanoma immunotherapy
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Authors : Wang et al., Nature Communications
2023-11 - An open label randomized controlled trial of atorvastatin versus aspirin in elite controllers and antiretroviral-treated people with HIV
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Authors : Mystakeli et al., AIDS
2023-10 - Effects of a Lacticaseibacillus Mix on Behavioural, Biochemical, and Gut Microbial Outcomes of Male Mice following Chronic Restraint Stress
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Authors : Letenneur et al., Nutrients
2023-10 - Hypomethylation-induced regulatory programs in T cells unveiled by transcriptomic analyses
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Authors : Lysandrou et al., Frontiers in Immunology
2023-09 - Pathogenetic associations of anti-ribosomal P protein antibody titres and their subclasses in patients with systemic lupus erythematosus
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Authors : Kaneko et al., Rheumatology
2023-08 - Intake of Lactiplantibacillus plantarum HEAL9 Improves Cognition in Moderately Stressed Subjects: A Randomized Controlled Study
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Authors : Onning et al., Nutrients
2023-08 - Tryptophan Modulation in Cancer-Associated Cachexia
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Authors : Agullo-Ortuno et al., International Journal of Molecular Sciences
2023-08 - Effects of Supplementation with the Standardized Extract of Saffron (affron®) on the Kynurenine Pathway and Melatonin Synthesis in Rats
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Authors : Munoz et al., Antioxydants
2023-07 - Advances in kynurenine analysis
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Authors : Mrstna et al., Clinica Chimica Acta
2023-07 - Atheroma plaque microenvironment stimulates kynurenine production by macrophages to induce endothelial adhesion molecules in the context of atherogenesis
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Authors : Dongen et al., bioRxiv
2023-07 - Involvement of the kynurenine pathway in breast cancer: updates on clinical research and trials
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Authors : Girithar et al., British Journal of Cancer
2023-04 - Tryptophan degradation enzymes expression in the placenta and the Kynurenine/Tryptophan ratio in maternal plasma after elective cesarean section
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Authors : Silvano et al., Journal of Reproductive Immunology
2023-02 - Citrulline and kynurenine to tryptophan ratio: potential EED (environmental enteric dysfunction) biomarkers in acute watery diarrhea among children in Bangladesh
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Authors : Das et al., Scientific Reports
2023-01 - Tryptophan degradation enzymes and Angiotensin (1−7) expression in human placenta
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Authors : Silvano et al., Journal of Reproductive Immunology
2022-09 - IDO1 is highly expressed in macrophages of patients in advanced tumour stages of oral squamous cell carcinoma
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Authors : Struckmeier et al., Journal of Cancer Reasearch & Clinical Oncology
2022-08 - Nociceptor-derived Reg3g prevents endotoxic death by targeting kynurenine pathway in microglia
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Authors : Sugisawa et al., Cell reports
2022-03 - Tryptophan Metabolism Is Associated with BMI and Adipose Tissue Mass and Linked to Metabolic Disease in Pediatric Obesity
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Authors : Lischka et al, Nutrients
2022-01 - Deep learning model enables the discovery of a novel immunotherapeutic agent regulating the kynurenine pathway
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Authors : Kim et al., Oncoimmunology
2021-11 - Circulating concentrations of citrulline, neopterin, kynurenine, and tryptophan during chemoradiation in patients with cervical carcinoma
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Authors : Pospíšková et al., Pteridines
2021-11 - Apathy is not associated with a panel of biomarkers in older adults with HIV disease
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Authors : Wood et al., Journal of psychosomatic research
2021-11 - Plasma Kynurenine-to-Tryptophan Ratio, a Highly Sensitive Blood-Based Diagnostic Tool for Tuberculosis in Pregnant Women Living With Human Immunodeficiency Virus (HIV)
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Authors : Adu-Gyamfi et al., Clinical infectious Diseases
2021-10 - The Role of Indoleamine 2,3-Dioxygenase in Renal Tubular Epithelial Cells Senescence under Anoxia or Reoxygenation
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Authors : Eleftheriadis et al., Biomolecules
2021-10 - The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus)
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Authors : Francikowski et al., Scientific reports
2021-10 - Abnormal Tryptophan Metabolism in HIV and Mycobacterium tuberculosis Infection
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Authors : Wang et al., Frontiers in microbiology
2021-06 - Decreased IDO1 Dependent Tryptophan Metabolism in Aged Lung during Influenza
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Authors : Jung Cho et al., European Respiratory Journal
2021-05 - Paliperidone Reversion of Maternal Immune Activation-Induced Changes on Brain Serotonin and Kynurenine Pathways
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Authors : MacDowell et al., Frontiers in Pharmacology
2021-05 - Role of indoleamine 2,3-dioxygenase in ischemia-reperfusion injury of renal tubular epithelial cells
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Authors : Eleftheriadis et al., Molecular Medicine Reports
2021-04 - Mood Disorder in Systemic Lupus Erythematosus Induced by Antiribosomal P Protein Antibodies Associated with Decreased Serum and Brain Tryptophan
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Authors : Cho et al., journal of Immunology
2021-03 - Early-Life Stress Modulates Gut Microbiota and Peripheral and Central Inflammation in a Sex-Dependent Manner
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Authors : Park et al., International journal of molecular sciences
2021-02 - Diagnostic accuracy of plasma kynurenine/tryptophan ratio, measured by enzyme-linked immunosorbent assay, for pulmonary tuberculosis
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Authors : Adu-Gyamfi et al., International journal of infectious diseases
2020-08 - Evidence that a deviation in the kynurenine pathway aggravates atherosclerotic disease in humans
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Authors : Baumgartner et al., Journal of internal medicine
2020-08 - Effect of repetitive transcranial magnetic stimulation on the kynurenine pathway in stroke patients
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Authors : Masachika et al., Clinical neuroscience
2020-06 - Prognostic value of indoleamine 2,3 dioxygenase in patients with higher-risk myelodysplastic syndromes treated with azacytidine
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Authors : Müller-Thomas et al., British journal of haematology
2020-04 - IDO Targeting in Sarcoma: Biological and Clinical Implications
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Authors : Nafia et al., Frontiers in immunology
2020-03 - Adding Indoximod to Hypofractionated Radiotherapy with Anti-PD-1 Checkpoint Blockade Enhances Early NK and CD8 + T-Cell-Dependent Tumor Activity
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Authors : Watanabe et al., Clinical Cancer Research
2019-11 - Blood Predictive Biomarkers for Patients With Non-small-cell Lung Cancer Associated With Clinical Response to Nivolumab
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Authors : Agullo-Ortuno et al., Clinical lung cancer
2019-08 - Traumatic injury leads to inflammation and altered tryptophan metabolism in the juvenile rabbit brain
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Authors : Zhang et al., Journal of Neurotrauma
2018-09 - Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice
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Authors : Walker et al., Molecular Psychiatry
2018-07 - Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats
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Authors : Martin-Hernandez et al., Molecular Neurobiology
2018-05 - Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections
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Authors : Ziklo et al., Frontiers in cellular and infection microbiology
2018-01 - In vivo inhibition of tryptophan catabolism reorganizes the tuberculoma and augments immune-mediated control of Mycobacterium tuberculosis
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Authors : Gautam et al., Proceedings of the National Academy of Sciences of the USA
2018-01 - Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas: A Phase 2 Clinical Trial
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Authors : Toulmonde et al., JAMA oncology
2018-01 - The Functional Relationship Between The Gut Microbiota And Generalized Anxiety Disorder in The Murine Model
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Authors : Perez Guzman et al., Thesis - McMaster University
2017-06 - In vitro rescue of genital strains of Chlamydia trachomatis from interferon-γ and tryptophan depletion with indole-positive, but not indole-negative Prevotella spp
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Authors : Ziklo et al. BMC Microbiology
2016-12
