L-Kynurenine ELISA kit
High Sensitivity

Ref: BA-E-2200
Diagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISAKynurenine pathwayTryptophan metabolism
DatasheetMSDSIVD Protocol RUO Protocol

The L-Kynurenine ELISA is an easy-to-use assay tailored for the quantification of Kynurenine across large series of samples. Optimized for the analysis of biological fluids (plasma, cell culture supernatant, serum…), the KYN ELISA features a lower limit of detection at 45.7ng/ml and a minimal sample volume of 20µl, thus allowing longitudinal studies in mice.

Sample typePlasma, serum, cell culture supernatant
Capacity96 tests
Sensitivity< 45.7ng/ml
Range100 – 10000ng/ml
Assay timeSample acylation 90 min, ELISA overnight
ReactivityReacts with all species
ReferencesCited in >50 papers
Size

1 x 96 wells

Regulatory status

In Vitro Diagnostics (EU), Research Use Only

492.00

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Total

492.00

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Product overview

Product nameL-Kynurenine ELISA kit
DescriptionEnzyme immunoassay (ELISA) for the quantitative determination of L-Kynurenine in biological fluids and cell supernatant
Format96-well plate
SamplesPlasma, serum, cell culture supernatant
Minimal sample volume20µL
ReactivityReacts with all species
Standard range0/100 – 10000ng/ml
Sensitivity

< 47.5ng/ml

SpecificityNo significant cross-reactivity was observed with L-Kynurenine analogs such as L-Tryptophan, 3-Hydroxy-DL-Kynurenine, 5-Hydroxy-DL-Tryptophan, Tyrosine, Serotonin, Phenylalanin, L-Asparagine and Kynurenic acid
Assay timeSample acylation 90 min and ELISA overnight
StorageStore at 2-8°C for to 6 months
DatasheetsInstructions for use, Material Safety Datasheet

 

Sample collection & storagePlasma/Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store smaples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)
Sample preparationSample acylation (90 min)
ELISAL-Kynurenine antiserum overnight incubation, revelation and read steps (1h)
Detailed protocolRUO: Download instructions for use

IVD:  Download instructions for use

 

Product citation

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    2023-07
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    Authors : Sugisawa et al., Cell reports
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    Authors : Lischka et al, Nutrients
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  • Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice
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    2017-07
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