Antibodies & immunoassays for small molecules L-Histidine Antibody – Rabbit Polyclonal
Ref: IS1066
Amino acidsPolyclonal AntibodiesICCIFIHC
+Affinity & specificity of anti-L-Histidine antibody
Competitive ELISA demonstrates that low amounts of L-Histidine conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Histamine, L-Histidinol, and 3-Methyl-L.Histidine (L-Histidine competitors) do not affect reaction (high specificity).
DatasheetMSDS
Highly affine and specific, the IS1066 anti-L-Histidine antibody can be used with the STAINperfect immunostaining kit A to perform ICC/IHC/IF stainings in cell cultures, whole mounts and tissue sections.
| Clonality | Polyclonal |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Validated by | ELISA |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µl |
Product overview
| Product name | L-Histidine – Rabbit pAb |
| Synonyms | Anti-Histidine antibody
Anti-His antibody Anti-2-Amino-3-(1H-imidazol-4-yl)propanoic acid antibody |
| Immunogen | Conjugated L-Histidine |
| Specificity | When tested in competitive ELISA, the anti-conjugated L-Histidine antibody did not show any significant cross-reactivity with L-Histidine competitors: Histamine, L-Histidinol, and 3-Methyl-L.Histidine |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage | Store at 4°C |
| Storage buffer | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
IF – Cell cultures, Whole mounts, Tissue sections
| Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample | |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
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