Antibodies & immunoassays for small molecules Kynurenic Acid/Quinolinic Acid ELISA pack – High Sensitivity
Ref: ISE-0102R
+Kynurenic Acid/Quinolinic Acid ELISA pack
Through the degradation of L-Tryptophan, the Kynurenine pathway (KP) generates a series of catabolites collectively known as Kynurenines, which can exert immunomodulatory and/or neuroactive properties. Known to respectively harbor neuroprotective and neurotoxic functions, Kynurenic (KYNA) and Quinolinic (QA) acids are found to be dysregulated in several neuroinflammatory disorders including Parkinson and Alzheimer disease but also Amyotrophic Lateral Sclerosis and Multiple Sclerosis.
Indended for research use only, the two kits included in the pack are ready to use, optimized for the quantitation of Kynurenic Acid (KYNA) and Quinolinic Acid (QA) within serum and plasma samples. These easy-to-use competitive assays are well-suited for both small and large series of samples. Working with a 25-50µL sample volume, these assays enable pre-clinical and clinical sample testings.
| Sample type | Serum, Plasma |
| Kit capacity | 2 x 96 tests |
| Sensitivity | LoD KYNA: 0.53ng/ml
LoD QA: 6ng/ml |
| Range | KYNA: 1.40 – 74ng/ml
QA: 25 – 200ng/ml |
| Reactivity | Reacts with all species |
| References | 5 papers with KYNA kit, 6 papers with QA kit |
Product overview
| Product name | Kynurenic Acid/Quinolinic Acid ELISA pack |
| Description | Two enzyme immunoassays (ELISA) allowing the quantitative determination of Kynurenic acid and Quinolinic acid in serum samples. For research use only |
| Labels | RUO |
| Format | 2 x 96-well plate |
| Samples | Serum, Plasma |
| Minimum sample volume | 25-50µL |
| Reactivity | Reacts with all species |
| Standard range | KYNA: 1.40 – 74ng/ml
QA: 25 – 2000ng/ml |
| Sensitivity |
LoD KYNA: 0.53ng/ml LoD QA: 6ng/ml |
| Specificity | No significant cross-reactivity was observed analogs for each kit. See product pages for Kynurenic acid ELISA and Quinolinic acid ELISA |
| Storage | Store at 2-8°C for to 6 months |
| Datasheets | Instructions for use – KYNA kit, Instructions for use – QA kit |
| Sample collection & storage | Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | KYNA: Sample derivatization (90 min)
QA: Sample acylation (120 min) |
| ELISA | Antisera overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Instructions for use – KYNA kit, Instructions for use – QA kit |
Product citations
- Atheroma plaque microenvironment stimulates kynurenine production by macrophages to induce endothelial adhesion molecules in the context of atherogenesis
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Authors : Dongen et al., bioRxiv
2023-07 - Advances in kynurenine analysis
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Authors : Mrstna et al., Clinica Chimica Acta
2023-07 - Unlocking the Secrets: Exploring the Biochemical Correlates of Suicidal Thoughts and Behaviors in Adults with Autism Spectrum Conditions
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Authors : Cremone et al., Biomedicines
2023-04 - Nociceptor-derived Reg3g prevents endotoxic death by targeting kynurenine pathway in microglia
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Authors : Sugisawa et al., Cell reports
2022-03 - Alzheimer’s-like signaling in brains of COVID-19 patients
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Authors : Reiken et al., Alzheimer's & Dementia
2022-02 - Apathy is not associated with a panel of biomarkers in older adults with HIV disease
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Authors : Wood et al., Journal of psychosomatic research
2021-11 - Electrochemical Determination of Kynurenine Pathway Metabolites—Challenges and Perspectives
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Authors : Sadok et al., Sensors
2021-10 - Synbiotic Supplementation Improves Obesity Index and Metabolic Biomarkers in Thai Obese Adults: A Randomized Clinical Trial
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Authors : Chaiyasut et al, Foods
2021-07
