Antibodies & immunoassays for small molecules Kynurenic Acid/Quinolinic Acid ELISA pack – High Sensitivity
Ref: ISE-0102R
+Kynurenic Acid/Quinolinic Acid ELISA pack
Through the degradation of L-Tryptophan, the Kynurenine pathway (KP) generates a series of catabolites collectively known as Kynurenines, which can exert immunomodulatory and/or neuroactive properties. Known to respectively harbor neuroprotective and neurotoxic functions, Kynurenic (KYNA) and Quinolinic (QA) acids are found to be dysregulated in several neuroinflammatory disorders including Parkinson and Alzheimer disease but also Amyotrophic Lateral Sclerosis and Multiple Sclerosis.
Indended for research use only, the two kits included in the pack are ready to use, optimized for the quantitation of Kynurenic Acid (KYNA) and Quinolinic Acid (QA) within serum and plasma samples. These easy-to-use competitive assays are well-suited for both small and large series of samples. Working with a 25-50µL sample volume, these assays enable pre-clinical and clinical sample testings.
| Sample type | Serum, Plasma |
| Kit capacity | 2 x 96 tests |
| Sensitivity | LoD KYNA: 0.53ng/ml
LoD QA: 6ng/ml |
| Range | KYNA: 1.40 – 74ng/ml
QA: 25 – 200ng/ml |
| Reactivity | Reacts with all species |
| References | Cited in 2 papers |
Product overview
| Product name | Kynurenic Acid/Quinolinic Acid ELISA pack |
| Description | Two enzyme immunoassays (ELISA) allowing the quantitative determination of Kynurenic acid and Quinolinic acid in serum samples. For research use only |
| Labels | RUO |
| Format | 2 x 96-well plate |
| Samples | Serum, Plasma |
| Minimum sample volume | 25-50µL |
| Reactivity | Reacts with all species |
| Standard range | KYNA: 1.40 – 74ng/ml
QA: 25 – 2000ng/ml |
| Sensitivity |
LoD KYNA: 0.53ng/ml LoD QA: 6ng/ml |
| Specificity | No significant cross-reactivity was observed analogs for each kit. See product pages for Kynurenic acid ELISA and Quinolinic acid ELISA |
| Storage | Store at 2-8°C for to 6 months |
| Datasheets | Instructions for use – KYNA kit, Instructions for use – QA kit |
| Sample collection & storage | Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | KYNA: Sample derivatization (90 min)
QA: Sample acylation (120 min) |
| ELISA | Antisera overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Instructions for use – KYNA kit, Instructions for use – QA kit |
Product citations
- Continuous theta burst stimulation for bipolar depression: A multicenter, double-blind randomized controlled study exploring treatment efficacy and predictive potential of kynurenine metabolites
Check the article
Authors : Dellink et al., Journal of Affective Disorders
2024-09 - The importance of oxidative stress and the kynurenine pathway of tryptophan metabolsm in the onset and development of multiple sclerosis in the population of Serbia
Check article
Authors : Marija Vasic, Thesis
2024-02
