Antibodies & immunoassays for small molecules Glycine Antibody – Rabbit Polyclonal
Ref: IS1034
NeuromediatorsAmino acidsPolyclonal AntibodiesICCIFIHC
+Glycine detection in primary mouse cortical neurons - fibers and soma
Primary mouse cortical neurons were stained with IS1034 anti-Glycine antibody using the STAINperfect immunostaining kit A and according to the optimized protocol for cell culture. After incubation with secondary antibody, this staining reveals the presence of Glycine within fibers and soma of some neurons. Image was obtained by confocal imaging.
DatasheetMSDS
This rabbit polyclonal anti-Glycine antibody was validated for IF in mouse primary cortical neurons in combination with the STAINperfect immunostaining kit A. Following sample preparation according to the kit, this antibody allows direct Glycine visualization in cell cultures, whole mounts and tissue sections.
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL (approx. 40 tissue sections) |
| References | Cited in 1 paper |
Product overview
| Product name | Glycine antibody – Rabbit polyclonal Ab |
| Synonyms | Anti-aminoacetic acid antibody |
| Immunogen | Conjugated Glycine |
| Specificity | When tested in competitive ELISA, the anti-conjugated Glycine antibody did not show any significant cross reactivity with Glycine analogs, including Alanine and Serine conjugates |
| Volume | 50µL |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for Glycine staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
Product citation
- INSIHGT: Accessible multimodal systems biology with quantitative molecular phenotyping in 3D
Check the article
Authors : Chun et al., bioRxiv
2024-06
