Antibodies & immunoassays for small molecules Free Metanephrine – Normetanephrine ELISA kit
Fast I Plasma samples
Ref: BA-E-8300
CatecholaminesDiagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISA
+ELISA kit for the detection of plasma metanephrines
DatasheetMSDSProtocol
This Free Metanephrines ELISA kit enables the fast measurement of free L-metanephrine and free L-Normetanephrine in plasma samples. Intended for in vitro diagnostics, this quantitative immunoassay is based on a simple and fast sample preparation step, and features a limit of detection below 25pg/ml.
| Sample type | 200µl plasma |
| Samples/kit | 80 samples – or 40 samples in duplicate |
| Sensitivity | 17pg/ml – Metanephrine 23pg/ml – Normetanephrine |
| Range | 36 – 3 600pg/ml – Metanephrine 72 – 7 200pg/ml – Normetanephrine |
| Assay time | Sample preparation and acetylation (30 min), ELISA overnight or 2h |
| Reactivity | Reacts with all species |
Product overview
| Product name | Metanephrine-Normetanephrine ELISA kit – Plasma – Fast-Track |
| Description | In vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of free metanephrine (metadrenaline) and free normetanephrine (Normetadrenaline) in plasma samples |
| Labels | IVD (EU), CE |
| Format | 96-well plate |
| Samples | Plasma |
| Sample volume | 200µL |
| Reactivity | Reacts with all species |
| Standard range | 0 / 36 – 3 600pg/ml – Metanephrine 0 / 72 – 7 200pg/ml – Normetanephrine |
| Sensitivity |
17pg/ml – Metanephrine |
| Specificity | No significant cross-reactivity was observed with Metanephrines analogs such as 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, VMS, HVMS, L-Dopa, L-Tyrosine, Tyramine and Acetaminophen |
| Assay time | Sample preparation and acetylation 30 min and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months |
| Datasheets | Instructions for use, Material safety datasheet |
| Sample collection & storage | EDTA- or Heparin-plasma sample only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C |
| Sample preparation | Sample precipitation and acetylation (30min) |
| ELISA | Metanephrine/Normetanephrine antisera incubation overnight or for 2h, revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
