Epinephrine – Norepinephrine ELISA kit – Fast – Plasma & Urine samples
Ref: BA-E-6500
DatasheetProtocolMSDS
This Epinephrine/Norepinephrine ELISA kit is tailored for the rapid measurement of Adrenaline (Epinephrine) and Noradrenaline (Norepinephrine) in urine and plasma samples. Intended for in vitro diagnostics and research use, this fast-track quantitative immunoassay enables to determine Epinephrine & Norepinephrine levels in up to 40 samples within 4 hours.
Sample type | 10µl urine, 300µl plasma |
Samples/kit | 40 samples in duplicate |
Sensitivity | Epinephrine: 0.3ng/ml urine, 10pg/ml plasma Norepinephrine: 1.5ng/ml urine, 50pg/ml plasma |
Range | Epinephrine: 1 – 200ng/ml Norepinephrine: 5 – 1 000ng/ml |
Assay time | Sample extraction and acylation (65 min), ELISA (3h) |
Reactivity | Reacts with all species |
Product overview
Product name | Epinephrine-Norepinephrine ELISA kit – Urine/Plasma – Fast-Track |
Description | In vitro diagnostics & Research enzyme immunoassay (ELISA) for the rapid determination of Adrenaline and Noradrenaline levels in plasma and urine samples |
Labels | IVD (EU Only) or RUO, CE |
Format | 2 x 96-well plate |
Samples | Urine, Plasma |
Sample volume | 10µl urine, 300µl plasma |
Reactivity | Reacts with all species |
Standard range | Epinephrine: 0 / 1 – 200ng/ml Norepinephrine: 0 / 5 – 1 000ng/ml |
Sensitivity |
Epinephrine: 0.3ng/ml urine, 10pg/ml plasma; Norepinephrine: 1.5ng/ml urine, 50pg/ml plasma |
Specificity | No significant cross-reactivity was observed with Epinephrine and Norepinephrine analogs such as Dopamine, Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid |
Assay time | 4 hours |
Storage | Store at 2-8°C for to 6 months |
Datasheets | Instructions for use, Material safety datasheet |
Sample collection & storage | Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C
Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C |
Sample preparation | Sample preparation, extractiion and acylation (65min) |
ELISA | Epinephrine/Norepinephrine antiserum incubation (2H), revelation and read steps (1h) |
Detailed protocol | Download instructions for use |
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