Epinephrine – Norepinephrine ELISA kit – Ultra-Sensitive – Any sample
Ref: BA-E-5400R
DatasheetProtocolMSDS
This research Epinephrine/Norepinephrine ELISA kit enables the ultra-sensitive measurement of Adrenaline (Epinephrine) and Noradrenaline (Norepinephrine) in any biological sample. Intended for research use only, the assay features a lower limit of detection at 6.6pg/ml and a minimal sample volume as low as 1µl.
Sample type | 1 – 750µl, all biological fluids |
Samples/kit | 40 samples in duplicate |
Sensitivity | Epinephrine: 6.6pg/ml; Norepinephrine: 2.6pg/ml |
Range | Epinephrine: 0.5 – 80ng/ml; Norepinephrine: 0.2 – 32ng/ml |
Assay time | Sample extraction, acylation and enzymatic conversion (4 h), ELISA overnight |
Reactivity | Reacts with all species |
Citations | Cited in 3 papers |
Product overview
Product name | Epinephrine-Norepinephrine ELISA kit – Research |
Description | Enzyme immunoassay (ELISA) for the ultra-sensitive determination of epinephrine and norepinephrine in biological samples. Research use only |
Format | 2 x 96-well plate |
Samples | Any sample |
Sample volume | 1 – 750µl |
Reactivity | Reacts with all species |
Standard range | Epinephrine: 0 / 0.5 – 80ng/ml; Norepinephrine: 0 / 0.2 – 32ng/ml |
Sensitivity |
Epinephrine: 6.6pg/ml; |
Specificity | No significant cross-reactivity was observed with Epinephrine and Norepinephrine analogs such as Dopamine, Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid |
Assay time | Sample preparation 4 hours and ELISA overnight |
Storage | Store at 2-8°C for to 6 months. |
Datasheets | Instructions for use, Material safety datasheet |
Sample preparation | Sample extraction and acylation (1h45), enzymatic conversion (2h) |
ELISA | Epinephrine/Norepinephrine antiserum overnight incubation, revelation and read steps (1h) |
Detailed protocol | Download instructions for use |
Product citations
- Upregulation of the hyperpolarization-activated current increases pacemaker activity of the sinoatrial node and heart rate during pregnancy in mice
Check the article
Authors : El Khoury et al., Circulation
2013-05 - Acute hypoxia induces hypertriglyceridemia by decreasing plasma triglyceride clearance in mice
Check the article
Authors : Jun et al., American journal of Physiology, Endocrinology & Metabolism
2012-05 - Pancreatic cancer cells and normal pancreatic duct epithelial cells express an autocrine catecholamine loop that is activated by nicotinic acetylcholine receptors α3, α5 and α7
Check the article
Authors : Al-Wadei et al., Molecular Cancer Research
2011-12