Antibodies & immunoassays for small molecules Citrulline-to-Arginine ratio ELISA pack – Serum, Plasma
Ref: ISE-0510R
Arginine metabolismAmino acidsResearch ELISA kits (RUO)ELISA
+Citrulline to Arginine ratio ELISA pack
Ref: ISE-0510R
DatasheetMSDSProtocol ArgProtocol Cit
Measuring the Citrulline-to-Arginine ratio is indicative of nitric oxide synthase (NOS) activity, which converts Arginine into Citrulline and Nitric Oxide (NO). A decreased plasma CIT-to-ARG ratio thus suggests high NO production, and may serve as a biomarker in metabolic and cardiovascular conditions.
| Sample type | 20 µl serum or plasma samples |
| Kit capacity | 2 x 96 tests |
| Sensitivity | ARG: 2.1µM
CIT: 1.47µM |
| Range | ARG: 5.12 – 200µM
CIT: 6.4 – 250µM |
| Reactivity | Reacts with all species |
Product overview
| Product name | Citrulline-to-Arginine ELISA pack |
| Description | Two enzyme immunoassays (ELISA) allowing the quantitative determination of L-Citrulline and L-Arginine in plasma/serum samples. For research use only |
| Labels | RUO |
| Format | 2 x 96-well plate |
| Samples | Serum, Plasma |
| Minimum sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | ARG: 5.12 – 200µM
CIT: 6.4 – 250µM |
| Sensitivity | ARG: 2.1µM
CIT: 1.47µM |
| Specificity | No significant cross-reactivity was observed with analogs for each kit. See product pages for Arginine ELISA and Citrulline ELISA |
| Storage | Store at 2-8°C for to 6 months |
| Datasheets | Instructions for use – Arg kit, Instructions for use – CIT kit |
| Sample collection & storage | Plasma: Use EDTA as anticoagulant
Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample preparation (3 hours) |
| ELISA | Antisera overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Instructions for use – Arg kit, Instructions for use – CIT kit |
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