Antibodies & immunoassays for small molecules Arginine-to-Ornithine ratio ELISA pack – Serum, Plasma
Ref: ISE-0410R
Arginine metabolismResearch ELISA kits (RUO)ELISA
+Arginine / Ornithine ELISA pack
ISE-0410R
DatasheetMSDS ArgProtocol ArgMSDS OrnProtocol Orn
Given the importance of Arginine metabolic pathway in physio-pathological processes and in particular in immune regulation, we developed and validated a robust and ease-to-use immunoassay for the simultaneous detection and quantification of both Arginine and Ornithine in plasma / sera originating from multiple species (20µL min. sample volume).
| Sample type | Serum, Plasma |
| Kit capacity | 2 x 96 tests |
| Sensitivity | Arg: 2.1µM
Orn: 8.5µM |
| Range | Arg: 5.12 – 200µM
Orn: 20 – 781.25µM |
| Reactivity | Reacts with all species |
Product overview
| Product name | Arginine/Ornithine ELISA pack |
| Description | Two enzyme immunoassays (ELISA) allowing the quantitative determination of L-Arginine and L-Ornitine in plasma/serum samples. For research use only |
| Labels | RUO |
| Format | 2 x 96-well plate |
| Samples | Serum, Plasma |
| Minimum sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | Arg: 5.12 – 200µM
Orn: 20 – 781.25µM |
| Sensitivity | Arg: 2.1µM
Orn: 8.5µM |
| Specificity | No significant cross-reactivity was observed with analogs for each kit. See product pages for Arginine ELISA and Ornithine ELISA |
| Storage | Store at 2-8°C for to 6 months |
| Datasheets | Instructions for use – Arg kit, Instructions for use – Orn kit |
| Sample collection & storage | Plasma: Use EDTA as anticoagulant
Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample preparation (3 hours) |
| ELISA | Antisera overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Instructions for use – Arg kit, Instructions for use – Orn kit |
Selected articles
- Caldwell et al., Arginase: A Multifaceted Enzyme Important in Health and Disease, Physiol Rev., 2018
- Niu et al., Arginase: An emerging and promising therapeutic target for cancer treatment, Biomed Pharmacother. 2022
- Apiz-Saab et al., Pancreatic tumors activate arginine biosynthesis to adapt to myeloid-driven amino acid stress, bioRxiv 2022
