3 Catecholamines ELISA kit
Fast – Plasma & Urine samples

Ref: BA-E-6600
CatecholaminesDiagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISA
DatasheetProtocolMSDS

This Catecholamine ELISA kit is tailored for the rapid measurement of Adrenaline (Epinephrine), Noradrenaline (Norepinephrine) and Dopamine in urine and plasma samples. Intended for in vitro diagnostics, this fast-track quantitative immunoassay enables to determine catecholamine levels in up to 40 samples within 4 hours.

Sample type10µl urine, 300µl plasma
Samples/kit40 samples in duplicate
SensitivityEpinephrine: 0.3ng/ml urine, 10pg/ml plasma Norepinephrine: 1.5ng/ml urine, 40pg/ml plasma Dopamine: 4.5ng/ml urine, 25pg/ml plasma
RangeEpinephrine: 1 – 200ng/ml
Norepinephrine: 5 – 1 000ng/ml
Dopamine: 4.5 – 2 000ng/ml
Assay timeSample extraction and acylation (65 min), ELISA (3h)
ReactivityReacts with all species
CitationsCited in 8 papers
Size

3 x 96 wells

Regulatory status

In Vitro Diagnostics (EU), Research Use Only

1,404.00

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1,404.00

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Product overview

Product name3 catecholamines ELISA kit – Urine/Plasma – Fast-Track
DescriptionIn vitro diagnostics (EU) & Research enzyme immunoassay (ELISA) for the rapid determination of Adrenaline, Noradrenaline and Dopamine levels in plasma and urine samples
LabelsIVD, CE, RUO
Format3×96-well plate
SamplesUrine, Plasma
Sample volume10µl urine, 300µl plasma
ReactivityReacts with all species
Standard rangeEpinephrine: 0 / 1 – 200ng/ml
Norepinephrine: 0 / 5 – 1 000ng/ml
Dopamine: 0 / 4.5 – 2 000ng/ml
Sensitivity

Epinephrine: 0.3ng/ml urine, 10pg/ml plasma Norepinephrine: 1.5ng/ml urine, 40pg/ml plasma Dopamine: 4.5ng/ml urine, 25pg/ml plasma

SpecificityNo significant cross-reactivity was observed with Dopamine, Epinephrine and Norepinephrine analogs such as Dopamine, Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid
Assay time4 hours
StorageStore at 2-8°C for to 6 months
DatasheetsInstructions for use, Material safety datasheet

 

Sample collection & storagePlasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C

Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15mL of 6M HCl. Storage: for longer periods (up to 6 months) at -20°C

Sample preparationSample preparation, extraction and acylation (65min)
ELISADopamine, Epinephrine, Norepinephrine antisera incubation (2H), revelation and read steps (1h)
Detailed protocolDownload instructions for use

Product citation

  • Doxorubicin Dose-Dependent Impact on Physiological Balance—A Holistic Approach in a Rat Model
    Check article
    Authors : Afonso et al., Biology
    2023-07
  • Antidepressant- and anxiolytic-like activities of Rosmarinus officinalis extract in rodent models: Involvement of oxytocinergic system
    Check the article
    Authors : Sasaki et al., Biomedicine & Pharmacotherapy
    2021-10
  • Effects of Sodium-Glucose Cotransporter 2 Inhibition on Glucose Metabolism, Liver Function, Ascites, and Hemodynamics in a Mouse Model of Nonalcoholic Steatohepatitis and Type 2 Diabetes
    Check the article
    Authors : Koichi et al., Journal of Diabetes Research
    2020-12
  • Resuscitation From Hemorrhagic Shock With Fresh and Stored Blood and Polymerized Hemoglobin
    Check the article
    Authors : Williams et al., Shock
    2020-10
  • Balance between oxygen transport and blood rheology during resuscitation 2 from hemorrhagic shock with polymerized bovine hemoglobin
    Check the article
    Authors : Williams et al., Journal of apllied physiology
    2020-07
  • Myogenic vasoconstriction requires G 12/G 13 and LARG to maintain local and systemic vascular resistance
    Check the article
    Authors : Chennupati et al., eLife
    2019-09
  • Increased arterial pressure in mice with overexpression of the ADHD candidate gene calcyon in forebrain
    Check the article
    Authors : Elmarakby et al., PloS one
    2019-02
  • Hyperfiltration in ubiquitin C-terminal hydrolase L1-deleted mice
    Check the article
    Authors : Boisvert et al., Clinical Science
    2018-07
  • Emotional states of love moderate the association between catecholamines and female sexual responses in the laboratory
    Check the article
    Authors : Dundon et al., Journal of sexual medicine
    2012-05
  • Renal kallikrein excretion and epigenetics in human acute kidney injury: expression, mechanisms and consequences
    Check the article
    Authors : Kang et al., BMC Nephrology
    2011-06

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