Antibodies & immunoassays for small molecules 25-OH Vitamin D ELISA kit I Fast I Serum and Plasma
Ref: MS-E-5800
EndocrinologyDiagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISA
+25-OH VITAMIN D ELISA-MS-E-5800
25-OH VITAMIN D ELISA-MS-E-5800
DatasheetMSDSProtocol (RUO)Protocol (IVD)
This 25-OH Vitamin D ELISA kit allows for the precise quantification of 25-hydroxyvitamin D in both plasma and serum samples. 25-OH Vitamin D, which is synthesized in the liver from vitamin D, is crucial for evaluating vitamin D deficiency, affecting bone health, immune function, and muscle function. The kit is suitable for both in vitro diagnostic and research purposes, featuring a 96-well plate format that supports high-throughput testing and ensures rapid results.
| Sample type | Serum and plasma |
| Capacity | 96 tests |
| Sensitivity | 3.224 ng/mL |
| Range | 0/5 – 120 ng/mL |
| Assay time | 105 min |
| Reactivity | Any species |
| Product name | 25-OH Vitamin D (total) ELISA |
| Description | In vitro diagnostics / Research enzyme immunoassay (ELISA) for the quantitative determination of total 25-OH Vitamin D in serum or plasma. |
| Labels | IVD (EU only) or RUO, CE |
| Format | 96-well plate |
| Samples | Serum and plasma |
| Sample volume | 50µL |
| Reactivity | Any species |
| Standard range | 0/5 – 120 ng/mL |
| Sensitivity | 3.224 ng/mL |
| Specificity | No significant cross-reactivity was observed with 25-OH Vitamin D2/D3 analogs |
| Assay time | 105 min |
| Product storage | Store at 2-8°C for up to 6 months |
| Sample collection & storage | Serum or plasma (EDTA, lithium heparin or citrate plasma): Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). Do not use hemolytic, icteric, or lipemic samples, and samples containing sodium azide. |
| Sample preparation | Release agent incubation (60min) Enzymatic conjugation (30 min) |
| ELISA | Incubation, revelation and read steps (15min) |
| Detailed protocol | IVD: Download instructions for use |