Antibodies & immunoassays for small molecules Serotonin Antibody – Goat Polyclonal
Ref: IS1035
NeuromediatorsTryptophan metabolismPolyclonal AntibodiesICCIFIHC
+Serotoninergic neurons in crayfish brain
Low magnification of serotoninergic neurons in the brain of a crayfish. This staining shows serotonin presence in soma and fibers. Tissue was processed following whole mount protocol of STAINperfect immunostaining kit A. Fluorescent labeled secondary antibody was used and image was obtained by fluorescence imaging.
DatasheetMSDS
The anti-Serotonin antibody IS1035 is a goat polyclonal antibody with high affinity and specificity. Using with the STAINperfect immunostaining kit A, our antibody stains directly 5-HT in whole mounts, cell culture and tissue sections.
| Clonality | Polyclonal antibody |
| Host | Goat |
| Reactivity | Reacts with all species |
| Tested samples | Whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL (approx. 40 tissue sections) |
Product overview
| Product name | Serotonin antibody (5-HT goat polyclonal Ab) |
| Synonyms | Anti-5-HT antibody
Anti-5-hydroxytryptamine antibody Anti-hydroxytryptamine antibody |
| Immunogen | Conjugated 5-HT |
| Specificity | When tested in competitive ELISA, the anti-conjugated Serotonin antibody did not show any significant cross reactivity with 5-Hydroxytryptamine analogs, including Tryptamine and 5-HydroxyTryptophan |
| Volume | 50µL |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage buffer | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for Serotonin staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
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