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Noradrenaline fibers in the pons of mouse embryo.Noradrenergic fibers were specificaly detected in the medulla of mouse embryo using STAINperfect immunostaining kit A and rabbit polyclonal anti-nordrenaline antibody and following the protocol for whole mount samples. Fluorescent secondary antibodies were used and pictures were acquired by confocal imaging.
Noradrenergic neurons in the pons of embryonic mouse.Using STAINperfect immunostaining kit A, the anti-Noradrenaline rabbit polyclonal antibody (Ref: IS1042) demonstrates the presence of noradrenaline in embryonic mouse pons (E13.5). The staining was performed according to the protocol optimized for whole-mount samples and was revealed using fluorescent conjugated secondary antibody.
Noradrenergic neurons in CNS of mouse embryo.Noradrenergic fibers were specifically detected in the medulla of mouse embryos using the STAINperfect Immunostaining Kit A in combination with a rabbit polyclonal anti-noradrenaline antibody, according to the protocol optimized for whole-mount samples. Fluorescently labeled secondary antibodies were applied, and images were acquired by confocal microscopy.
Norepinephrine fibers in the spinal cord of embryonic mouseNoradrenergic fibers were specifically detected in the medulla of mouse embryos using the STAINperfect Immunostaining Kit A combined with a rabbit polyclonal anti-noradrenaline antibody, following the manufacturer's recommended protocol for whole-mount samples. Fluorescent secondary antibodies were employed, and images were acquired using confocal microscopy.
Affinity & specificity of anti- L-Noradrenaline antibodyCompetitive ELISA demonstrates that low amounts of L-DOPA conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of L-DOPA competitors Dopamine or Normetanephrine do not affect reaction (high specificity).
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Rodent brain, adipose & spinal cord tissues, and spider brain tissues. |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
What is the L-Noradrenaline (Norepinephrine) Rabbit Polyclonal Antibody (IS1042)?
A rabbit-derived polyclonal primary antibody that specifically binds noradrenaline (norepinephrine) with high affinity and specificity, validated using competitive ELISA. It shows minimal cross-reactivity with dopamine and normetanephrine.
For which applications and sample types has this antibody been validated?
This antibody has been successfully validated for immunofluorescence (IF) in mouse brain and spinal cord tissues, particularly in embryonic mouse pons (E13.5), using the STAINperfect™ Immunostaining Kit A. It has also been used to visualize noradrenergic neurons in spider brain (Jin et al., Nature Ecology & Evolution, 2023) and to detect norepinephrine in urothelial tissues (e.g., bladder mucosa) using IF.
What is the format and quantity of the antibody?
It is supplied as a purified liquid polyclonal antibody in a 50 µL vial, sufficient for approximately 40 tissue-section stainings.
Does it cross-react with related catecholamines?
The antibody works in any species species. It was successfully applied to rodent brain, adipose tissue, spinal cord, and spider brain tissues. Notably, it's also been successfully applied to urothelial tissues in the bladder.
Which staining kit should I use for optimal results?
Use the STAINperfect™ Immunostaining Kit A, which offers optimized protocols for cell cultures, whole-mounts, and tissue sections.
What protocols and sample preparations are recommended?
The STAINperfect kit includes detailed protocols tailored for whole-mounts, cell cultures, and tissue sections. Always follow the manufacturer’s instructions for best results.
How should the antibody be stored?
According to available protocols for ImmuSmol antibodies: store at +4 °C for short-term use (1–2 months), and aliquot and freeze at –20 °C for long-term storage. Avoid repeated freeze–thaw cycles.
Is this antibody intended for clinical or diagnostic use?
No—this product is for research use only and is not approved for diagnostic or therapeutic applications.
How is it shipped?
Immusmol offers worldwide shipping via FedEx International Priority® and provides a worry-free warranty.
L-Noradrenaline Antibody (Norepinephrine) – Rabbit Polyclonal
Anti-L-Noradrenaline (NA) antibody IS1042 was validated for immunofluorescence in mouse brain and spinal cord tissues, in combination with the STAINperfect immunostaining kit A. This anti-Norepinephrine antibody was recently used to evidence noradrenergic neurons in spider brain (Jin et al, Nature Ecology & Evolution, 2023).
€ 449
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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
Direct customer support is available all business days.
A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online.
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IS1042
L-Noradrenaline Antibody (Norepinephrine) – Rabbit Polyclonal
Anti-Noradrenaline pAb – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | L-Noradrenaline antibody – Rabbit pAb |
| Synonyms | Anti-Noradrenaline antibody Anti-Norepinephrine antibody Anti-NA antibody |
| Immunogen | Conjugated Noradrenaline |
| Specificity | When tested in competitive ELISA, the anti-conjugated NA antibody did not show any significant cross reactivity with for example Dopamine and normetanephrine conjugates. |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
