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STAINperfect™ – Trial pack
Immunostaining of glutamatergic cells in the crayfish brain.Anti-L-Glutamate antibody highlights the presence of glutamatergic cells into the brain of a crayfish. Staining was obtained with anti-L-Glutamate antibody and performed with STAINperfect immunostaining kit A, following the protocol for whole mounts samples. Alexa Fluor® 488 conjugated secondary antibody was used and images obtained by confocal imaging at high magnification.
Glutamatergic cell populationin a mature rat cortical culture revealed by L-glutamate immunoreactivity detection using rabbit polyclonal (#IS1001) antibodies, with the STAINperfect immunostaining kit A.
Glutamatergic cells in brain stem of mouse embryo ( E13.5).Immunostaining of L-Glutamate cells in the brain stem of E13.5 mouse embryon following whole mount protocol provided with STAINperfect immunostaining kit A.Our optimized L-Glutamate rabbit antibody allowed L-Glutamate detection with a cytoplasmic pattern. Secondary antibody (Alexa Fluor® 488 conjugated) was used and staining visualized by confocal imaging.
L-Glutamate (green) and MAP2 (red) immunostaining of mouse cortical primary neurons cultureImmunodetection of L-Glutamate (green) and MAP2- (red) positive neurons in mouse primary cortical culture. Staining was performed using STAINperfect immunostaining kit A, according to the protocol optimized for cell culture. After addition of a fluorescent labeled secondary antibody this staining reveals the presence of L-Glutamate within fibers and soma of neurons.
Low magnification of embryonic (E15.5) medulla immunostaining of glutamatergic cells.Glutamatergic cells into the hindbrain of mouse embryo (E15.5) were labeled with antibodies to L-Glutamate using STAINperfect immunostaining kit A and the protocol for whole mounts. Goat anti-rabbit Alexa Fluor® 488 secondary antibody was used and picture was acquired by confocal imaging.
Affinity & specificity of anti-L-Glutamate antibodyCompetitive ELISA demonstrates that low amounts of L-Glutamate conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of D-Glutamate, L-Glutamine and L-Aspartate conjugates do not affect reaction (high specificity).
L-Glutamic acid (L-Glutamate)Amino acid L-Glutamic acid (L-Glutamate) is the major excitatory neurotransmitter in the vertebrate nervous system. Agonist of NMDA, AMPA, Kainate and metabotropic receptors, L-Glutamic acid regulates synaptic plasticity, and is thus involved in learning and mnemonic processes. However, by activating NMDA receptors, L-Glutamic acid may also lead to neuronal damage and death. Glutamate toxicity is thus associated with the pathogenesis of neurodevelopmental and neurodegenerative disorders.
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Rodent primary neurons and brain tissues, crayfish tissues |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
What is L-Glutamate Antibody IS1001 ?
The L-Glutamate Antibody IS1001 is a rabbit polyclonal antibody with high affinity and strong specificity toward L-glutamate. It minimizes cross-reactivity with related compounds, ensuring reliable detection in immunohistochemistry and immunofluorescence studies.
What are the main applications ?
IS1001 has been validated for immunofluorescence and immunohistochemistry. It is effective in rodent primary neurons, mouse brain tissues, crayfish brain tissues, and other neuronal models. When combined with the STAINperfect immunostaining kit A, it enables precise visualization of glutamate in cell cultures, tissue sections, and whole mounts.
How specific is this antibody ?
The L-Glutamate Antibody IS1001 shows high specificity for L-glutamate. Validation by competitive ELISA confirms negligible cross-reactivity with D-glutamate, L-glutamine, and L-aspartate, ensuring accurate staining of glutamatergic pathways.
How should IS1001 be stored ?
This rabbit polyclonal antibody is supplied as lyophilized purified antiserum. Once reconstituted, it can be kept at +4 °C for up to two weeks or stored long term at −20 °C in aliquots. To maintain performance, repeated freeze-thaw cycles should be avoided.
Does it work across species ?
Yes, IS1001 works across species and has been successfully applied to g rodent and crayfish neuronal tissues.
Is IS1001 for clinical use ?
No, IS1001 is strictly intended for research use. It is not approved for diagnostic or therapeutic applications.
L-Glutamate Antibody – Rabbit Polyclonal
The anti-L-Glutamate antibody IS1001 has been validated for immunofluorescence applications in rodent primary neurons, mouse brain tissues, and crayfish brain tissues, in combination with the STAINperfect immunostaining kit A. Recent publications have leveraged this antibody to effectively stain glutamatergic systems in both mouse tissues and primary neurons.
€ 449
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IS1001
L-Glutamate Antibody – Rabbit Polyclonal
Anti-L-Glutamate pAb – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | L-Glutamate antibody – Rabbit polyclonal Ab |
| Synonyms | Anti-L-Glutamic acid antibody |
| Immunogen | Conjugated L-Glutamate |
| Specificity | When tested in competitive ELISA, the anti-conjugated L-Glutamate antibody did not show any significant cross reactivity with L-Glutamic acid analogs, including D-Glutamate, L-Glutamine and L-Aspartate conjugates |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
