The clear path to small molecule visualization & quantification

Key features

Clonality Monoclonal antibody
Host Mouse
Reactivity Reacts with all species
Tested samples Mouse primary neurons, human iPSC-derived neurons
Staining procedure  STAINperfect immunostaining kit A
(protocols for cell cultures, whole mounts, tissue sections)
Format 50µL (approx. 40 tissue sections)

Citations

Frequently asked questions

L-Glutamate Antibody IS018 is a mouse monoclonal antibody designed to detect L-Glutamic acid with high specificity and affinity. It shows minimal cross-reactivity with D-Glutamate or L-Aspartate.

This antibody is species-independent and has been validated in mouse primary neurons as well as human iPSC-derived neurons.

IS018 is optimized for immunofluorescence and works particularly well when combined with the STAINperfect immunostaining kit A. It is suitable for use on cell cultures, whole mounts, and tissue sections.

The recommended dilution range is 1:250 to 1:1000 when using the diluent provided in STAINperfect kit A. The protocol should be adapted to the type of sample being analyzed

For short-term use, the antibody should be stored at +4 °C. For long-term storage, it should be aliquoted and kept at –20 °C, avoiding repeated freeze–thaw cycles.

Each vial contains 50 µL of purified monoclonal antibody, which is typically enough for staining around 40 tissue sections.

Yes, this antibody is available for order. It comes with technical support, including direct access to the scientist who developed it, and is backed by quality guarantees.

Yes. L-Glutamate Antibody IS018 is strictly for research use only and is not intended for diagnostic or therapeutic applications.

Yes. It has been used in peer-reviewed studies, including a 2019 publication where it was applied to stain L-Glutamate in human iPSC-derived neurons.

The STAINperfect immunostaining kit A provides optimized buffers and protocols that enhance the specificity and reproducibility of L-Glutamic acid labeling, ensuring consistent and accurate results across different sample types.

IS018

Glutamate Antibody (L) – Mouse Monoclonal

The anti-L-Glutamate antibody IS018 was validated in mouse primary neurons in combination with the STAINperfect immunostaining kit A. A recent paper also used this mouse monoclonal antibody to stain for L-Glutamate in human iPS-derived neurons.

 449

Number of citations :

79 in stock

79 in stock

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Not getting satisfactory results ? If product did not meet quality standards, customer support offers replacement or refund.

For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using. 

Direct customer support is available all business days.

A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online. 

They published with this product

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IS018

Glutamate Antibody (L) – Mouse Monoclonal

Anti-L-Glutamate mAb – Purified monoclonal antibody for research use only (liquid 50µL)

Specifications

Product name L-Glutamate antibody – mouse mAb
Synonyms Anti-L-Glutamic acid antibody
Immunogen Conjugated L-Glutamate
Isotype
IgG1 k chain
Clone
Clone 1D51B1
Specificity When tested in competitive ELISA, the anti-conjugated Glutamate antibody did not show any significant cross reactivity with its analogs, including D-Glutamate
Volume 50 µL
Form Liquid
Purity Purified IgG

Instructions

Storage
Store at +4°C for short term (6 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles
Immunofluorescence (IF)
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution.
Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections)
Comments Optimal working dilutions must be determined by the end-user
Restrictions For research use only

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