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Imaging of L-DOPA neurons in mouse primary neuronsOur rabbit polyclonal anti-L-DOPA antibody - IS1030 - allows the detection of L-DOPA neurons in primary mouse neurons. Staining was performed using STAINperfect immunostaining kit A, according to the protocol optimized for cell culture. Fluorescent labeled secondary antibody was used and picture was acquired by high-content imaging. Such staining highlights the presence of L-DOPA both in soma and neurons fibers.
Immunofluorescence imaging of L-DOPA in crayfishL-DOPA is detected within fibers with varicosities within the fourth thoracic ganglia of crayfish. Staining was performed using STAINperfect immunostaining kit A following the protocol dedicated to whole mount samples. Secondary antibody (goat anti-rabbit Alexa Fluor® 488) was used and picture was acquired by confocal imaging at high magnification.
Affinity & specificity of anti- L-DOPA antibodyCompetitive ELISA demonstrates that low amounts of L-DOPA conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of L-DOPA competitors Dopamine or Normetanephrine do not affect reaction (high specificity).
L-DOPAOur rabbit polyclonal anti-L-DOPA antibody - IS1030 - allows the detection of L-DOPA neurons in primary mouse neurons. Staining was performed using STAINperfect immunostaining kit A, according to the protocol optimized for cell culture. Fluorescent labeled secondary antibody was used and picture was acquired by high-content imaging. Such staining highlights the presence of L-DOPA both in soma and neurons fibers.
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Mouse primary neurons and crayfish tissues |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
L-DOPA Antibody – Rabbit Polyclonal
The anti-L-DOPA antibody IS1030 was validated for immunofluorescence in crayfish tissues and mouse primary neurons using the STAINperfect immunostaining kit A, demonstrating specific and accurate labeling of L-DOPA.
€ 449
92 in stock
| Save with quantity discounts | Unit price after discount |
|---|---|
| 3 to 5 antibodies (any reference) | € 399 |
| 6+ antibodies (any reference) | € 379 |
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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
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A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online.
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IS1030
L-DOPA Antibody – Rabbit Polyclonal
Anti-L-DOPA pAb – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | L-DOPA antibody – Rabbit pAb |
| Synonyms | Anti-3,4-Dihydroxy-L-phenylalanine antibody |
| Immunogen | Conjugated L-DOPA |
| Specificity | When tested in competitive ELISA, the anti-conjugated L-DOPA antibody did not show any significant cross reactivity with L-Serine analogs, including Normetanephrine and Dopamine conjugates. |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
