Metanephrine-Normetanephrine ELISA kit – Fast – Urine samples

Ref: BA-E-8600
Research ELISA kits (RUO)ELISACatecholaminesDiagnostic ELISA kits (IVD)
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Urine Metanephrines ELISA
DatasheetProtocolMSDS

This Metanephrines ELISA kit enables to quantify metanephrine and normetanephrine in urine samples within 2 hours. Intended for in vitro diagnostics & Research, this quantitative immunoassay demonstrated good correlation with HPLC.

Sample type 25µl urine
Samples/kit 40 samples in duplicate
Sensitivity Metanephrine: 13 ng/ml
Normetanephrine: 23 ng/ml
Range Metanephrine: 20 – 2 000 ng/ml
Normetanephrine: 30 – 3 000ng/ml
Assay time Sample hydrolysis and acetylation (45 min), ELISA (1h)
Reactivity Reacts with all species
Citations Cited in 1 paper
Size

2 x 96 wells
Regulatory status

In Vitro Diagnostics (EU), Research Use Only

579.00€

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Quantity
Total

579.00€

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Product overview

Product name Metanephrine-Normetanephrine ELISA kit – Urine- Fast-Track
Description In vitro diagnostics & research enzyme immunoassay (ELISA) for the fast determination of metanephrines (metadrenaline and normetadrenaline) in urine samples
Labels IVD (EU only) or RUO, CE
Format 96-well plate
Samples Urine
Sample volume 25µL
Reactivity Reacts with all species
Standard range 0 / 20 – 2 000ng/ml – Metanephrine
0 / 30 – 3 000ng/ml – Normetanephrine
Sensitivity

13ng/ml – Metanephrine
23ng/ml – Normetanephrine
Specificity No significant cross-reactivity was observed with Metanephrines analogs such as 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, Vanillic mandelic acid, L-Dopa, Homovanillic acid, L-Tyrosine and Tyramine
Assay time Sample hydrolysis and acetylation 45 min and ELISA
Storage Store at 2-8°C for to 6 months
Datasheets Instructions for use, Material safety datasheet

 

Sample collection & storage Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage at -20°C. for long periods (up to 6 months)
Sample preparation Sample hydrolysis (30 min) and acetylation (15min)
ELISA Metanephrine antiserum incubation (30 min), revelation and read steps (30h)
Detailed protocol Download instructions for use

Product citation

  • Quantification of normetanephrine in canine urine using ELISA: evaluation of factors affecting results
    Check the article
    Authors : Hoglund et al., Journal of veterinary diagnosic investigation
    2021-10

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