3 Catecholamines ELISA kit
Fast – Plasma & Urine samples
Ref: BA-E-6600
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Plasma/Urine catecholamines ELISA kit: Dopamine, Adrenaline, Noradrenaline
DatasheetProtocolMSDS
This Catecholamine ELISA kit is tailored for the rapid measurement of Adrenaline (Epinephrine), Noradrenaline (Norepinephrine) and Dopamine in urine and plasma samples. Intended for in vitro diagnostics, this fast-track quantitative immunoassay enables to determine catecholamine levels in up to 40 samples within 4 hours.
Sample type | 10µl urine, 300µl plasma |
Samples/kit | 40 samples in duplicate |
Sensitivity | Epinephrine: 0.3ng/ml urine, 10pg/ml plasma Norepinephrine: 1.5ng/ml urine, 40pg/ml plasma Dopamine: 4.5ng/ml urine, 25pg/ml plasma |
Range | Epinephrine: 1 – 200ng/ml Norepinephrine: 5 – 1 000ng/ml Dopamine: 4.5 – 2 000ng/ml |
Assay time | Sample extraction and acylation (65 min), ELISA (3h) |
Reactivity | Reacts with all species |
Citations | Cited in 8 papers |
They published with this product see papers
Product overview
Product name | 3 catecholamines ELISA kit – Urine/Plasma – Fast-Track |
Description | In vitro diagnostics (EU) & Research enzyme immunoassay (ELISA) for the rapid determination of Adrenaline, Noradrenaline and Dopamine levels in plasma and urine samples |
Labels | IVD, CE, RUO |
Format | 3×96-well plate |
Samples | Urine, Plasma |
Sample volume | 10µl urine, 300µl plasma |
Reactivity | Reacts with all species |
Standard range | Epinephrine: 0 / 1 – 200ng/ml Norepinephrine: 0 / 5 – 1 000ng/ml Dopamine: 0 / 4.5 – 2 000ng/ml |
Sensitivity |
Epinephrine: 0.3ng/ml urine, 10pg/ml plasma Norepinephrine: 1.5ng/ml urine, 40pg/ml plasma Dopamine: 4.5ng/ml urine, 25pg/ml plasma |
Specificity | No significant cross-reactivity was observed with Dopamine, Epinephrine and Norepinephrine analogs such as Dopamine, Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid |
Assay time | 4 hours |
Storage | Store at 2-8°C for to 6 months |
Datasheets | Instructions for use, Material safety datasheet |
Sample collection & storage | Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C
Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15mL of 6M HCl. Storage: for longer periods (up to 6 months) at -20°C |
Sample preparation | Sample preparation, extraction and acylation (65min) |
ELISA | Dopamine, Epinephrine, Norepinephrine antisera incubation (2H), revelation and read steps (1h) |
Detailed protocol | Download instructions for use |
Product citations
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2020-07 - Myogenic vasoconstriction requires G 12/G 13 and LARG to maintain local and systemic vascular resistance
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Authors : Chennupati et al., eLife
2019-09 - Increased arterial pressure in mice with overexpression of the ADHD candidate gene calcyon in forebrain
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2019-02 - Hyperfiltration in ubiquitin C-terminal hydrolase L1-deleted mice
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Authors : Boisvert et al., Clinical Science
2018-07