Antibodies & immunoassays for small molecules L-Glutamine Antibody – Rabbit Polyclonal
Ref: IS1060
ICCIFIHCAmino acidsPolyclonal Antibodies
+Affinity & specificity of anti- L-Glutamine antibody
Competitive ELISA demonstrates that low amounts of L-Glutamine conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of L-Ornithine, L-Arginine and L-Asparagine (L-Glutamine competitors) do not affect reaction (high specificity).
DatasheetMSDS
This rabbit polyclonal antibody recognizes L-Glutamine at a 1/10 000 working dilution, while displaying no significant cross-reactivity with competitors. Using the STAINperfect immunostaining kit A, this primary antibody was used to visualize Glutamine in midbrain organoids (IF) and human tumor tissues (IHC).
| Clonality | Polyclonal |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Validated application | IF/IHC |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µl |
| References | Cited in 2 papers |
Product overview
| Product name | L-Glutamine – Rabbit pAb |
| Synonyms | Anti-L-Glutamine antibody
Anti-Levoglutamide antibody Anti-L-(+)-Glutamine antibody Anti-Glutamic acid amide antibody Anti-(S)-2,5-Diamino-5-oxopentanoic acid antibody |
| Immunogen | Conjugated L-Glutamine |
| Specificity | When tested in competitive ELISA, the anti-conjugated L-Ornithine antibody did not show any significant cross reactivity with L-Ornithine competitors: L-Ornithine, L-Arginine and L-Asparagine |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage | Store at 4°C |
| Storage buffer | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
IF – Cell cultures, Whole mounts, Tissue sections
| Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample | |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
Product citations
- Monocarboxylate Transporter-1 (MCT1)-Mediated Lactate Uptake Protects Pancreatic Adenocarcinoma Cells from Oxidative Stress during Glutamine Scarcity Thereby Promoting Resistance against Inhibitors of Glutamine Metabolism
Check the article
Authors : Ammar et al., Antioxydants
2023-09 - Single-cell transcriptomics reveals multiple neuronal cell types in human midbrain-specific organoids
Check the article
Authors : Smits et al., Cell & tissue research
2020-07
