Antibodies & immunoassays for small molecules L-Arginine Antibody – Rabbit Polyclonal
Ref: IS1055
Arginine metabolismAmino acidsPolyclonal AntibodiesICCIFIHC
+Affinity & specificity of anti-Arginine antibody
Competitive ELISA demonstrates that low amounts of L-Arginine conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of L-Ornithine, L-Glutamate and L-Homoarginine (L-Arginine competitors) do not affect reaction (high specificity).
DatasheetMSDS
Validated by direct and competitive ELISA, this rabbit polyclonal antibody recognizes L-Arginine at 1/10 000 working dilution, while displaying no significant cross-reactivity with competitors such as L-Ornithine, L-Glutamate and L-Homoarginine. Our team is currently working on the IF/ICC/IHC protocol for optimal staining in combination with the STAINperfect immunostaining kit A.
| Clonality | Polyclonal |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Validated by | ELISA |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL |
Product overview
| Product name | L-Arginine – Rabbit pAb |
| Synonyms | Anti-L-alpha-amino-delta-guanidinovaleric acid antibody
Anti-1-Amino-4-guanidovaleric acid antibody Anti-Levargin antibody |
| Immunogen | Conjugated L-Arginine |
| Specificity | When tested in competitive ELISA, the anti-conjugated L-Homoarginine antibody did not show any significant cross reactivity with L-Arginine competitors, L-Ornithine, L-Glutamate and L-Homoarginine |
Storage
| Form | Liquid |
| Purity | Purified antiserum |
| Storage | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
Optimization of staining protocol ongoing
| Standard STAINperfect procedure | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
Antibody not yet cited.
