Metanephrine ELISA kit
Fast – Urine samples
Ref: BA-E-8400
DatasheetMSDSProtocol
This Metanephrine ELISA kit enables to quantify metanephrine in urine samples within 2 hours. Intended for in vitro diagnostics & research use, this quantitative immunoassay demonstrated good correlation with HPLC (see product data sheet).
Sample type | 25µl urine |
Samples/kit | 40 samples in duplicate |
Sensitivity | 13ng/ml |
Range | 20 – 2 000ng/ml |
Assay time | Sample hydrolysis and acetylation (45 min), ELISA 1h |
Reactivity | Reacts with all species |
Citations | Cited in 1 paper |
Product overview
Product name | Metanephrine ELISA kit – Urine- Fast-Track |
Description | In vitro diagnostics & Research enzyme immunoassay (ELISA) for the fast determination of metanephrine (metadrenaline) in urine samples |
Labels | IVD (EU Only) or RUO, CE |
Format | 96-well plate |
Samples | Urine |
Sample volume | 25µL |
Reactivity | Reacts with all species |
Standard range | 0 / 20 – 2 000ng/ml |
Sensitivity |
13ng/ml |
Specificity | No significant cross-reactivity was observed with Metanephrine analogs such as Normetanephrine, 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, Vanillic mandelic acid, L-Dopa, Homovanillic acid, L-Tyrosine and Tyramine |
Assay time | Sample hydrolysis and acetylation 45 min and ELISA 1h |
Storage | Store at 2-8°C for to 6 months |
Datasheets | Instructions for use, Material safety datasheet |
Sample collection & storage | Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage at -20°C. for long periods (up to 6 months) |
Sample preparation | Sample hydrolysis (30 min) and acetylation (15min) |
ELISA | Metanephrine antiserum incubation (30 min), revelation and read steps (30h) |
Detailed protocol | Download instructions for use |
Product citation
- Quantification of normetanephrine in canine urine using ELISA: evaluation of factors affecting results
Check the article
Authors : Hoglund et al., Journal of veterinary diagnosic investigation
2021-10