GABA Antibody – Chicken Polyclonal
Ref: IS1036![GABA and L-Serine in mouse primary neurons culture](https://www.immusmol.com/wp-content/uploads/2022/03/gaba594-l-ser488-x40live_2-4-2016_4-35-19_pm_dsp_791.jpg)
GABA and L-Serine in mouse primary neurons culture
Primary mouse cortical neurons were stained using anti-GABA chicken polyclonal antibody (red) and anti-L-Serine polyclonal rabbit antibody (green) using the STAINperfect immunostaining kit A and according to the optimized protocol for cell culture. Co-immunostaining appears in yellow. Alexa fluor 546 and 488 conjugated secondary antibodies respectively were used and picture obtained by confocal imaging.
DatasheetMSDS
The anti-GABA antibody IS1036 is a chicken polyclonal antibody developed for its superior affinity and specificity but also to work in multiplex staining with rabbit polyclonal or mouse monoclonal antibodies. Combined with the STAINperfect immunostaining kit A for samples preparation, this antibody allows visualization of GABA in whole mounts, cell cultures and tissue sections.
Clonality | Polyclonal |
Host | Chicken |
Eligible samples | Whole mounts, cell culture, tissue sections |
Reactivity | Reacts with all species |
Staining procedure | STAINperfect immunostaining kit A |
Format | 50µL (approx. 40 tissue sections) |
References | Cited in 4 papers |
Product overview
Product name | GABA antibody – Chicken pAb |
Synonyms | Anti-gamma-Aminobutyric acid antibody |
Immunogen | Conjugated GABA |
Specificity | When tested in competitive ELISA, the anti-conjugated GABA antibody did not show any significant cross reactivity with gamma-Aminobutyric acid analogs, including beta-Alanine and D-Alanine |
Volume | 50µL |
Lot number | 150901 |
Expiration date | 2025-09-01 |
Storage
Form | Liquid |
Purity | Purified anti-serum |
Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
Material safety datasheet | Download MSDS |
IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
Comments | Optimal working dilutions must be determined by the end-user |
Restrictions | For research use only |
Full protocol | Download STAINperfect protocol for GABA staining |
Protocols-at-a-glance |
Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
Product citations
- INSIHGT: Accessible multimodal systems biology with quantitative molecular phenotyping in 3D
Check the article
Authors : Chun et al., bioRxiv
2024-06 - Gad1 knock-out rats exhibit abundant spike-wave discharges in EEG, exacerbated with valproate treatment
Check the article
Authors : Liu et al., Frontiers in Immunology
2023-09 - Early-life experiences altered the maturation of the lateral habenula in mouse models, resulting in behavioural disorders in adulthood
Check the article
Authors : Nakamura et al., Journal of Psychiatry & Neuroscience
2021-08 - Impact of Sleep-Wake-Associated Neuromodulators and Repetitive Low-Frequency Stimulation on Human iPSC-Derived Neurons
Check the article
Authors : Yokoi et al., Frontiers in neuroscience
2019-05