Challenge Serotonin and its precursor L-Tryptophan using STAINperfect immunostaining kit

Using STAINperfect immunostaining kit A, Tryptophan was detected within the hindbrain of embryonic mouse E15.5 following the protocol for whole mount samples (image on left, red). Serotonin, a tryptophan metabolite, was detected in the raphe nucleus from adult mice on floating sections prepared with the STAINperfect immunostaining kit A. Stainings were performed using anti-Tryptophan Mouse monoclonal Ab (IS011) and Serotonin Rabbit polyclonal Ab (IS1007). Fluorescent conjugated secondary antibodies were used and images acquired by confocal imaging.

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