Levels of dopamine highlighted by ELISA in the afferent and efferent dopaminergic systems
Figure 1: Mouse brain tissue levels of dopamine quantified and shown in different areas using a specific dopamine ELISA kit (BA-E-5300). As expected, high levels of dopamine were detected in the afferent and efferent dopaminergic systems, i.e. putamen, midbrain, and olfactory bulbs.
Dopaminergic nigral neurons identified by IS1005 Dopamine antibody that colocalizes with TH staining
Figure 2: Dopaminergic nigral neurons in coronal rat brain sections revealed by anti-dopamine (DA, rabbit polyclonal antibody #IS1005) and Tyrosine Hydroxylase (TH, #MAB5280, reference catecholaminergic neuron immunostaining). As highlighted in the overlay, DA immunoreactivity localizes with TH staining in the Substantia nigra pars compacta, thus showing that our antibody against the DA neurotransmitter – used with the STAINperfect immunostaining kit A – is a validated tool to directly highlight DAergic system networks.
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